The success of your chromatin immunoprecipitation (ChIP) experiment depends on the fragmentation of chromatin, a critical step in the ChIP protocol. This can be accomplished with either sonication or enzymatic digestion. But how do you decide which chromatin fragmentation protocol to use in your ChIP experiments? A number of factors can influence your choice, making a decision seem daunting. So let’s simplify – watch the video and we'll will show you the way!
In this video, we’ll walk through four questions that will help you find a protocol and the ideal ChIP kit from CST that’s suited to your experimental needs.
- What is your input sample type, cells or tissues?
- What type of protein is being targeted for IP - histones, transcription factors, or cofactors?
- Is your target protein high or low abundance?
- What's your preferred method for chromatin fragmentation - sonication or enzymatic digestion?
In many instances, you can use either sonication or enzymatic digestion, but in the video we will also cover some scenarios in which one method for chromatin fragmentation outperforms the other.
Once you've answered these questions and are ready to set up your experiment, click below to find your kit!