CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology® (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

All Posts

4 Situations Where Flow Cytometry Might Be More Useful Than Western Blotting

Western blotting is used to detect proteins and analyze their expression using antibodies, but it does have its limitations. Flow cytometry also employs antibodies, but it uses fluorescence to detect and analyze protein expression on a cell-by-cell basis.

This video describes four situations where it can be worth going with flow over western blot: 1) Analysis of multiple targets (multiplexing), 2) analysis of cell subpopulations 3), intracellular signaling events, 4) correlative analysis.

 

Learn more about the benefits of using flow.

Download our Signaling Flow Cytometry guide.

Chris S
Chris S
Chris Sumner is the Editor-in-Chief of Lab Expectations. When he's not reading/writing about curing disease, he's hiking in the woods, playing guitar, or searching for the world's best lobster roll.

Related Posts

Tools for Modulating Cells

Cell behavior is a tightly regulated, finely tuned process where various different signals work together ...
Andrea T Oct 13, 2021 3:00:00 AM

Tools for Monitoring Cell State

Have you been wondering how to monitor a cell state or cellular response? From a basic research standpoin...
Andrea T Oct 6, 2021 3:00:00 AM

The Importance of Using a Validated Antibody Pair in a High-throughput Immunoassay

Over the years there have been numerous additions and variations to the original ELISA format including e...
Mark S Sep 29, 2021 3:00:00 AM