CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

4 Situations Where Flow Cytometry Might Be More Useful Than Western Blotting

Read More
All Posts

Western blotting is used to detect proteins and analyze their expression using antibodies, but it does have its limitations. Flow cytometry also employs antibodies, but it uses fluorescence to detect and analyze protein expression on a cell-by-cell basis.

This video describes four situations where it can be worth going with flow over western blot: 1) Analysis of multiple targets (multiplexing), 2) analysis of cell subpopulations 3), intracellular signaling events, 4) correlative analysis.

 

Learn more about the benefits of using flow.

Download our Signaling Flow Cytometry guide.

Chris Sumner
Chris Sumner
Chris Sumner was the Editor-in-Chief of Lab Expectations. When he's not reading/writing about curing disease, he's hiking in the woods, playing guitar, or searching for the world's best lobster roll.

Related Posts

Employee-Directed Global Giving Provides $150K for Humanitarian Causes

At Cell Signaling Technology (CST), our philosophy of giving and supporting those in need is at the heart...
Krystyna Hincman Mar 20, 2024

Five Reasons to Choose a Conjugation Service Over an Antibody Labeling Kit

The need for antibody conjugation is on the rise due to the increased use of multiplexing. Not only are e...
Alexandra Foley Mar 6, 2024

Networking at Conferences: Five Tips for the Introverted Scientist

Networking is an important part of any research career,1 with many experts considering networking and net...
Kenneth Buck, PhD Feb 28, 2024