3 Questions to ask before starting your next immunofluorescence experiment.


Posted by Carolyn P on Sep 7, 2016 3:00:00 AM

Specificity, consistency, and optimized assay conditions are three key elements that help ensure reliable immunofluorescence (IF) staining results each and every time. So, before starting an experiment you should ask yourself the following three questions:

  • Is your antibody specific?
  • Is your antibody supported by an optimized IF protocol?
  • Is your antibody performing consistently?

Let's take them each in turn...

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Topics: Protocols, Primary Antibodies, Antibody Validation, IF-IC, Cell Biology, techniques

Roundtable Discussion on Antibody Validation


Posted by Carolyn P on Feb 17, 2016 3:00:00 AM

Antibody Validation Roundtable: Specificity + Sensitivity + Reproducibility = Validation 

Concerns about the use of incorrectly or insufficiently characterized antibodies in biomedical research have been raised by recent reports of nonreproducible research studies. Non-reproducible research studies have resulted in loss of time, resources, and scientific reputation, with publication retractions occurring in certain cases.

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Topics: Antibody Performance, Primary Antibodies, Antibody Validation, Webinars, Reproducibility

Simplifying Proteomics - Part 3


Posted by Claire S on Dec 9, 2015 3:00:00 AM

Part 1 and part 2 introduced mass spectrometry-based proteomic methods, like PTMScan®, for the study of post-translational modifications (PTMs). In the final part of this series you can see how PTMScan can be applied in translational research.

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Topics: Primary Antibodies, Post Translational Modification, Proteomics

Simplifying Proteomics - Part 2


Posted by Claire S on Dec 2, 2015 3:00:00 AM

Part 1 gave an overview on mass spectrometry-based proteomics. Now it’s time to talk about how this strategy can be used to identify peptides with post-translational modifications (PTM) from a complex biological sample.

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Topics: Primary Antibodies, Post Translational Modification, Proteomics

Why High Concentration doesn't always mean good Antibody Performance.


Posted by Carolyn P on Jul 29, 2015 5:00:00 AM

If you buy primary antibodies from different places, you may have noticed that many companies list the size of their products in micrograms per ml (i.e., by weight). You may have also noticed that CST does not - CST antibodies are listed by performance (e.g., 10 western blots) instead.

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Topics: Antibody Performance, Primary Antibodies, techniques

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