Webinar - Multiplex Immunohistochemistry (IHC)

Posted by Liana G on Jan 13, 2016 3:00:00 AM

Analysis of Immune Checkpoint Control Protein Co-expression in Breast and Ovarian Cancer Using Novel Rabbit Monoclonal Antibodies and Multiplex IHC

With an increasing number of biomarkers and, often, limited availability of biopsy material, there is a growing need for multiplexed assays for both research and clinical purposes. IHC based solutions are particularly attractive in the field of immuno-oncology, as maintaining spatial context within the tumor microenvironment provides meaningful and potentially actionable information.

Watch the video below.



Topics: Protocols, IHC, Cancer Immunology, Cancer Research, mIHC

Journal Club:  Hippo pathway and intestinal stem cell regeneration

Posted by Liana G on Jan 6, 2016 3:00:00 AM

Journal Club: YAP-dependent reprogramming of Lgr5+ stem cells drives intestinal regeneration and cancer.

In the spirit of keeping you abreast of new and interesting scientific discoveries, the writers at CST have embraced the idea of a virtual journal club, where we discuss recently published scientific literature.


Topics: Cancer Research, Journal Club

Webinar - Advancements in AMPK and Autophagy Signaling

Posted by Liana G on Nov 18, 2015 3:30:00 AM

Reuben Shaw, Ph.D

Professor, Molecular and Cell Biology Laboratory, Deputy Director, Salk Cancer Center, The Salk Institute for Biological Studies

AMPK, a highly conserved sensor of cellular energy status, is found in all eukaryotic cells and maintains metabolic homeostasis by reprogramming growth, metabolism, and autophagy in the face of cellular stresses. AMPK is activated by direct binding of AMP and ADP to its regulatory subunits, which enhances its phosphorylation by the upstream kinase LKB1, a tumor suppressor gene frequently inactivated in sporadic human lung and cervical cancer.


Topics: Metabolism, Cell Biology

POSTER | Tumor Immunology: Examining the Microenvironment

Posted by Liana G on Oct 21, 2015 5:30:00 AM

The immune system is tasked with distinguishing ‘self’ from ‘non-self’. Tumors can, quite often, be highly immunogenic as a result of their mutational burden that can make them appear less like ‘self’.


Topics: Cancer Immunology, Just for fun

Immunotherapy: Empowering Nature’s Anti-tumor Artillery - Part II

Posted by Liana G on Sep 9, 2015 8:00:00 AM

At your last visit we talked about the rapidly rising popularity of cancer immunotherapy and how it relies on capitalizing on our own immune system to eradicate malignant growth. 

We discussed the benefits of immune checkpoint blockade involving neutralizing monoclonal antibodies as a successful strategy in unmasking cancer cells, thus making them vulnerable to immune attack. So successful, in fact, that cancer immunotherapy based on this strategy was named ‘breakthrough of the year’ in 2013.

Having said that, not all patients respond to checkpoint blockade. Hence, there is much room for improvement and a pressing need for alternative approaches and better characterized biomarkers that can help stratify patients and make more accurate predictions on the prognostic outcome of a given treatment.

So, what are some of the advancements made in developing additional immunotherapeutic strategies to tackle cancer?


Topics: Cancer Immunology

Immunotherapy: Empowering Nature’s Anti-Tumor Artillery - Part I

Posted by Liana G on Sep 2, 2015 9:31:00 AM

If you happened to attend AACR, AAI, or ASCO this year you’d agree that immune cell-based cancer therapies have caused quite the stir. Immunotherapy as an anti-neoplastic strategy has gradually claimed the spotlight over the past several years. In fact, some now consider it as one of the pillars of cancer therapy along with chemotherapy, radiation, surgery, and targeted therapy. Scientists and clinicians alike are cautiously optimistic, and point to response rates never seen before. 

And, why not take advantage of our own immune system and the best of its weaponry, the T cells, to fight cancer?


Topics: Cancer Immunology

Intracellular Flow Cytometry in Action

Posted by Liana G on Aug 12, 2015 8:00:00 AM

Traditionally, flow cytometry has been used to identify distinct cell types within a heterogeneous pool of cells, based on extracellular or surface marker expression, an application commonly known as immuno-phenotyping. However, this technology is also readily amenable to intracellular target detection and can be successfully applied to the study of complex signaling events.


Topics: Protocols, Flow, Cell Biology, techniques

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